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KMID : 0380620210530030344
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 3 p.344 ~ p.347
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In vitro hepatocyte inflammation by chaparral extract
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Kim Il-Rang
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Abstract
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In this study, the hepatotoxic mechanism of chaparral (Larrea tridentata) was investigated through in vitro experiments that measured cell death, inflammatory cytokine secretion, and intracellular fat accumulation by treating HepG2 hepatocytes with a 70% ethanol extract of chaparral at concentrations ranging from 0.001 to 100 μg/mL. Cell death was observed after treatment with chaparral extract at concentrations of 1-100 μg/mL (p<0.05). The secretion of the inflammatory cytokines, interleukin-8 and macrophage-colony stimulating factor, and fat accumulation were significantly increased even at a concentration of 0.1 μg/mL, which was 10 times lower than the observed concentration resulting in cell death (p<0.05). Hepatitis caused by inflammatory cytokine secretion and fat accumulation was shown to be a form of hepatotoxicity induced by chaparral extract. Hepatitis was expressed at a concentration lower than that causing serious toxicity such as cell death, suggesting that hepatotoxicity, including hepatitis, may be caused by ingestion of low concentrations of chaparral.
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KEYWORD
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chaparral (Larrea tridentata), hepatotoxicity, hepatitis, cytokine, lipid accumulatio
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